Determination of Biological Activity of Hylocereus polyrhizus Methanol Extracts: Antimicrobial Activity, Probiotic-Promoting Effect, Photoprotective Activity
Abstract
In our study, the biological activity of Hylocereus polyrhizus obtained from Türkiye was determined. Firstly, antimicrobial activity of methanol extracts obtained from H. polyrhizus against test microorganisms was determined by disc diffusion susceptibility assay, minimum inhibition concentration (MIC) and minimum fungicidal or bactericidal concentration (MFC or MBC) tests. Then, viable cell counting against Limosilactobacillus fermentum MA-7 using the macro-dilution method was used to determine the probiotic-promoting effect of the fruit extracts. Finally, the photo-protective activity of the extracts and extract-cream mixtures was obtained spectrophotometrically. The inhibition zone diameter of the peel extract against the test microorganisms is in the range of 9.60-12.37 mm and the fruit extract is in the range of 7.85-12.00 mm. MBC values ranged from 40 µg/µl to 80 µg/µl in peel extract and between 10 µg/µl and >80 µg/µl in the fruit extract. The inhibition zone diameters of the extracts against probiotics were determined as 6.10 -10.25 mm. As a result of the viable cell count, the viability rate of L. fermentum MA-7 increased as the fruit methanol extract concentration and time increased. The photoprotective activities of the peel and fruit extracts were determined as 13.17 and 12.26. It was determined that the extracts increased sun protection factor (SPF) value of the cream. The results indicated that H. polyrhizus extracts may have potential for use in the nutraceutical products, cosmetic and pharmaceutical industries.
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H. Jiang, W. Zhang, X. Li, C. Shu, W. Jiang, and J. Cao, “Nutrition, phytochemical profile, bioactivities and applications in food industry of pitaya (Hylocereus spp.) peels: A comprehensive review,” Trends in Food Science & Technology, vol. 116, pp. 199-217, 2021.
H. Song, Q. Chu, D. Xu, Y. Xu. and X. Zheng, “Purified betacyanins from Hylocereus undatus peel ameliorate obesity and insulin resistance in high-fat-diet-fed mice,” Journal of Agricultural and Food Chemistry, vol. 64, no. 1, pp. 236-244, 2016.
N.S. Ramli, L. Brown, P. Ismail, and A. Rahmat, “Effects of red pitaya juice supplementation on cardiovascular and hepatic changes in high-carbohydrate, high-fat diet-induced metabolic syndrome rats,” BMC Complementary and Alternative Medicine, vol. 14, no. 1, pp. 1-10, 2014.
M. Joshi, and B. Prabhakar, “Phytoconstituents and pharmaco‐therapeutic benefits of pitaya: A wonder fruit,” Journal of Food Biochemistry, vol. 44, no. 7, e13260, 2020.
J.R. Zgoda, and J.R. Porter, “A convenient microdilution method for screening natural products against bacteria and fungi,” Pharmaceutical Biology, vol. 39, no. 3, pp. 221–225, 2001.
A.S. Rusdianto, A. Yulianti, S. Suwasono, and A.E. Wiyono, “The characteristics of liquid soap with additional variations of moringa seed extract (Moringa oleifera L.),” International Journal on Food, Agriculture and Natural Resources, vol. 2, no. 3, pp. 5-11, 2021.
D. Savoia, “Plant-derived antimicrobial compounds: alternatives to antibiotics,” Future Medicine, vol. 7, no. 8, pp. 979–990, 2012.
S.R. Shankar, R. Rangarajan, D.V.L. Sarada, and C.S. Kumar, “Evaluation of antibacterial activity and phytochemical screening of Wrightia tinctoria L.,” Pharmacognosy Journal, vol. 2, no. 14, pp. 19-22, 2010.
A.G. Cruz, R.S. Cadena, E.H. Walter, A.M. Mortazavian, D. Granato, J.A. Faria, and H.M. Bolini, “Sensory analysis: relevance for prebiotic, probiotic, and synbiotic product development,” Comprehensive reviews in food science and food safety, vol. 9, no. 4, pp. 358-373, 2010.
J.A. Krumbeck, M.X. Maldonado-Gomez, A.E. Ramer-Tait, and R.W. Hutkins, Prebiotics and synbiotics: dietary strategies for improving gut health,” Current opinion in gastroenterology,; vol. 32, no. 2, pp. 110-119, 2016.
D. Davani-Davari, M. Negahdaripour, I. Karimzadeh, M. Seifan, M. Mohkam, S.J. Masoumi, A. Berenjian, and Y. Ghasemi, “Prebiotics: definition, types, sources, mechanisms, and clinical applications,” Foods, vol. 8, no. 3, pp. 92, 2019.
A. Moongngarm, N. Trachoo, and N. Sirigungwan, “Low molecular weight carbohydrates, prebiotic content, and prebiotic activity of selected food plants in Thailand,” Advance Journal Food Science and Technology, vol. 3, no. 4, pp. 269-274, 2011.
N.S. Oh, J.Y. Lee, J.Y. Joung, K.S. Kim, Y.K. Shin, K.W. Lee, S.W. Kim, S.N. Oh, and Y. Kim, “Microbiological characterization and functionality of set-type yogurt fermented with potential prebiotic substrates Cudrania tricuspidata and Morus alba L. leaf extracts,” Journal of dairy science, vol.99, no. 8, pp. 6014-6025, 2016.
D. Lin, M. Xiao, J. Zhao, Z. Li, B. Xing, X. Li, M. Kong, L. Li, Q. Zhang, Y. Liu, H. Chen, W. Qin, H.C. Wu, and S. Chen, “An overview of plant phenolic compounds and their importance in human nutrition and management of type 2 diabetes,” Molecules, vol. 2, no. 10, 1374, 2016.
S.C. Costa, C.B. Detoni, C.R. Branco, M.B. Botura, and A. Branco, “In vitro photoprotective effects of Marcetia taxifolia ethanolic extract and its potential for sunscreen formulations,” Revista Brasileira de Farmacognosia, vol. 25, no. 4, pp. 413-418, 2015.
F.P.D. Souza, G.R. Campos, and J.F. Packer, “Determinação da atividade fotoprotetora e antioxidante em emulsões contendo extrato de Malpighia glabra L.–Acerola,” Revista de Ciências Farmacêuticas Básica e Aplicada, vol. 34, no. 1, pp. 69-77, 2013.
F. Rodrigues, I. Almadia, B. Sarmento, M.H. Amaral, and M.B.P.P Oliveira, “Study of the Isoflavone Content of Different Extracts of Medicago spp. as Potential Active Ingredient,” Industrial Crops Products, vol. 57 pp. 110-115, 2014.
R.K. Chaudhuri, “Modulating Antioxidant Defenses to Enhance Skin Benefits,” Euro Cosmetics, View Publication Stats, pp. 20-24, 2015.
R. Sousa, C. Antunes, and F. Guilhermino, “Factors influencing the occurrence and distribution of Corbicula fluminea (Müller, 1774) in the River Lima estuary,” In Annales de Limnologie-International Journal of Limnology, vol. 42 no. 3 pp. 165-171, 2006.
J.D.S. Mansur, M. Breder, M. Mansur, and R.D. Azulay, “Determination of sun protection factor by spectrophotometry,” An Bras Dermatol, vol. 61 no. 3 pp. 121-124, 1986.
R.M. Sayre, P.P. Agin, G.J. LeVee, and E. Marlowe, “A comparison of in vivo and in vitro testing of sunscreening formulas,” Photochemistry and Photobiology, vol. 29, no. 3, pp. 559-566, 1979.
M. Asan-Ozusaglam, and I. Celik, “White pitahaya as a natural additive: potential usage in cosmetic industry,” Foods and Raw Materials, vol. 11, no. 1, pp. 57-63, 2023.
A.L. Medina, M.E. Lucero, F.O. Holguin, R.E. Estell, J.J. Posakony, J. Simon, M.A. O'Connell, “Composition and antimicrobial activity of Anemopsis californica leaf oil,” Journal of Agricultural and Food Chemistry, vol. 53, no. 22, pp. 8694-8698, 20025.
C.D. Romero, S.F. Chopin, G. Buck, E. Martinez, M. Garcia, and L. Bixby, “Antibacterial properties of common herbal remedies of the southwest,” Journal of ethnopharmacology, vol. 99, no. 2, pp. 253-257, 2005.
M.A.K Rohin, C.A. Abu Bakar, and A.M. Ali, “Antibacterial activity of flesh and peel methanol fractions of red pitaya, white pitaya and papaya on selected food microorganisms,” International Journal of Pharmacy and Pharmaceutical Sciences, vol. 4, no. 3, pp. 185-190, 2012.
E.M. Altuner, and B. Cetin, “Antimicrobial Activity of Thuidium delicatulum (Bryopsida) Extracts,” Kafkas University Institute of Science and Technology Journal, vol. 2, no. 2, pp. 85-92, 2009.
I. Diyatri, W.S Juliastuti, R.D. Ridwan, G.C. Ananda, F.A. Waskita, N.V. Juliana, S.P. Khansa, R.T. Pratiwi, and C.R. Putri, “Antibacterial effect of a gingival patch containing nano-emulsion of red dragon fruit peel extract on Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, and Fusobacterium nucleatum assessed in vitro,” Journal of Oral Biology and Craniofacial Research, vol. 13, no. 3, pp. 386-391, 2023.
Y.Y. Yong, G. Dykes, S.M. Lee, and W.S. Choo, “Comparative study of betacyanin profile and antimicrobial activity of red pitahaya (Hylocereus polyrhizus) and red spinach (Amaranthus dubius),” Plant Foods for Human Nutrition, vol. 72, pp. 41-47, 2017.
B. Seckin, I. Turkan, A.H. Sekmen, and C. Ozfidan, “The role of antioxidant defense systems at differential salt tolerance of Hordeum marinum Huds.(sea barleygrass) and Hordeum vulgare L.(cultivated barley),” Environmental and Experimental Botany, vol. 69. No. 1, pp. 76-85, 2010.
S.G.G. Morais, G.D.S.C. Borges, M. dos Santos Lima, O. Martín-Belloso, and M. Magnani, “Effects of probiotics on the content and bioaccessibility of phenolic compounds in red pitaya pulp,” Food Research International, vol. 126, 108681, 2019.
K. Naghmouchi, Y. Belguesmia, F. Bendali, G. Spano, B.S. Seal, and D. Drider, “Lactobacillus fermentum: A bacterial species with potential for food preservation and biomedical applications,” Critical reviews in food science and nutrition, vol. 60, no. 20, pp. 3387-3399, 2020.
S. Wichienchot, M. Jatupornpipat, R.A. Rastall. “Oligosaccharides of pitaya (dragon fruit) flesh and their prebiotic properties.” Food chemistry, vol. 120 no. 3, pp. 850-857, 2010.
S. Gajardo, M. Aguilar, T. Stowhas, F. Salas, J. Lopez, C. Quispe, P.B. Calderon, and J. Benites, “Determination of sun protection factor and antioxidant properties of six Chilean Altiplano plants,” Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas, vol. 25, no. 5, pp. 352-36, 2016.
G. Janarny, S. Ranaweera, and P.P. Gunathilake, “Response surface optimization of extraction parameters for enhanced phenolic extraction and antioxidant activity from Cassia fistula flowers,” International Journal on Food, Agriculture and Natural Resources, vol. 4, no. 1, pp. 1-9, 2023.
R. Vijayakumar, S.S. Abd Gani, U.H. Zaidan, M.I.E. Halmi, T. Karunakaran, and M.R. Hamdan, “Exploring the potential use of Hylocereus polyrhizus peels as a source of cosmeceutical sunscreen agent for its antioxidant and photoprotective properties,” Evidence-Based Complementary and Alternative Medicine, 7520736, 2020.
N.V. Yanishlieva, E. Marinova, and J. Pokorný, “Natural antioxidants from herbs and spices,” European Journal of Lipid Science and Technology, vol. 108, no. 9, pp. 776-793, 2006.
R.R. Korać, and K.M. Khambholja, “Potential of herbs in skin protection from ultraviolet radiation,” Pharmacognosy Review, vol. 5, no. 10, pp. 164-173, 2011.
DOI: https://doi.org/10.46676/ij-fanres.v5i3.344
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