Comparative Studies on The Efficacy of Some Fungicides Activities on Fungal Seed Borne Pathogens of Commercial Maize Seeds


Egbo Nwakaku Jane, Ugoh Sylvester Chukwudi, Olisa Babafemi Sunday


Abstract


This study investigated the efficacy of some fungicides activities on fungal seed borne pathogens of two commercial maize seeds (Sammaz 15 and Sammaz 52). A total of 2500 seeds were randomly picked for each variety to represent a working sample from the seed reference collections. Out of the 2500 seeds, 400 seeds were surface–sterilized with 10% sodium hypochlorite solution, cultured and examined microscopically for the presence of fungal pathogens. Molecular analysis confirmed that all isolated pathogens matched with the reference accession from NCBI molecular database. Seeds were treated with different dosages of fungicides viz Thiram and Metalaxy + Tebuconazole and incubated for 5 days. The effect of the various fungicides on the incidence of seed- borne fungi, effect on seed germination and interaction of the fungicide were evaluated and analyzed. Percentage occurrence of the fungal pathogens revealed that Sammaz 15 have F. solani (29.0%), A. flavus (20.3%), A. niger (19.0%) and Rhizpus stolanifera (15.0%). In Sammaz 52, A.niger (40.0%), A. flavus (19.7%) and F. solani (27.0%). It was observed that F. solani was predominant in sammaz 15 while A. niger was predominant in Sammaz 52. Thiram (45.88 mm) at all doses showed greater inhibitory effects on mycelia growth than Metalaxyl + Tebucanozole (24.25 mm). The correlation analysis performed  on the data set reveals that  there is a  negative significant  correlation between  zone of  inhibition  and percent  disease  incidence  (r = -0.99***), but the relationship was positive with number of non-infected seeds (r = 0.99***). This shows that increasing the zone of inhibition will increase the number of non-infected seeds and the normal seedling (germination percentage). Thiram fungicides observed to be more effective in reducing the disease incidence than Metalaxyl fungicides.


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References


Akonda Moshiur Rahman, Md., Yasmin, M., Hossain, I. Incidence of seed-borne mycoflora and their effects on germination of maize seed. International. Journal of Agricultural Science and Research. 8 (1), 87 -92. 2016.

Amodu, U.S. and Aku, B.O. Seed-Borne Diseases and Nigeria Agriculture. Scholars Journal of Agriculture and Veterinary Science. 2(3B):243-252.2015.

Anjorin, S.T. and Mohammed, M. Effect of seed borne fungi on germination and Seedling vigour of watermelon (Citrullus lonatus thumb. African Journal of Plant Science. 8(5). Pp. 232-326.2014.

Basra, A.S. Handbook of Seed Science and Technology, Food Product Press, New York. Pp 150. 2006.

Datta, S., Dey, P., Sarkar, A., Tarafdar, J. and Chowdhury, A. Comparison among Four triazole fungicides on growth and development of sheath blight of rice pathogen Rhizoctonia solani Kühn AG1-1A, Archives of Phytopathology and Plant Protection. 49 (1): 239 – 251. 2016.

Fagundes, L.K., Nunes, U.R., Prestes, O.D., Fernandes, T.S., Ludwig, E.J., Saibt, N. Rice Seed Treatment and Recoating with Polymers: Physiological Quality and Retention of Chemical Products. Rev. Caatinga, Mossoró. 30 (4): 920 – 927. 2017.

Fawole, O.B., Ahmed, O. and Adetunji, S.B. Detection and determination of pathogenicity of seed-borne fungi in maize seeds varieties. Science Focus 15(2): 249-252. 2010.

Galperin, M., Graf, S., David, K. Seed Treatment prevents vertical transmission of Fusarium moniliforme making a significant contribution to disease control. Phytoparasitica. 31 (4): 344-352. 2003.

ISTA, ISTA Handbook on Seedling Evaluation. Third Edition with Amendment. International Seed Testing Association, Bassersdorf, Switzerland. 2001.

Kator, L., Aondo, T.O., and Akinyemi, B.K. Isolation and Identification of seed borne fungi of common bean (Phaseolus Vulgaris L.) from selected Markets in Makurdi. International Journal of Applied Agricultural Sciences 2(5): 75-78. 2016.

Lević, J., Stanković, S., Krnjaja, V., Bočarov-Stančic, A., and Ivanovic, D. Distribution frequency and incidence of seed-borne pathogens of some cereals and industrial crops in Serbia. Pestic. Phytomed. (Belgrade), 27 (1), 2012, 33-40. 2012.

Machado de Matos, C.de.S, Barrocas, E.N., Machado J. da C., and Alves, F. C. Health and physiological quality of corn seeds treated with fungicides and assessed during storage. Journal of Seed Science, 35(1): 10-16. 2013.

Mathur, S.B. and Kongsdal, O. Common Laboratory Seed Health Testing method for detecting fungi. First Edition. International Seed Testing. Pp. 5-10. 2003.

McGee, D.C. Maize Disease. A Reference source for seed Technologists. APS Press, St. Paul, Minnesota. Pp. 1-20. 1998.

Omaima, A.H., Hassan, M.S., Amal, S.H and Ahmed, S. Isolation and Molecular identification of Fusarium fungi from some Egyptian Grains. Asia Journal of Plant Sciences. 17(4): 182-190. 2018.

Parween, T., Jan, S., Mahmooduzzafar, S., Fatma T., and Zahid Hameed Siddiqui, Z.H. Selective Effect of Pesticides on Plant—A Review, Critical Reviews in Food Science and Nutrition, 56(1): 160-179. 2016.

Pedro, W.C., David, L.H., and Michael, J.W. Identifying and naming plant Pathogenic fungi: Present and future. Animal Review of Phytopathology. 53 (1): 247-267. 2015.

Rashid, M. H., Ashraf Hossain, M., Kashem, M. A., Shiv KumarRafii , M. Y. and Latif, M. A. Efficacy of Combined Formulations of Fungicides with Different Modes of Action in Controlling Botrytis Gray Mold Disease in Chickpea. The Scientific World Journal. Pp. 1-6. 2014.

Rodriguez, E., Chevalie, J., El Ghoul. H., Voldum-Clausen, K., Mundy, J., Petersen, M. DNA damage as a consequence of NLR activation. PLOS Genetics. 14(2): 100 – 150. 2018.

Rodriguez-Brljevich C. Interaction of fungicide seed treatments and the Fusarium-Maize (Zea mays L.) pathosystem. 204: 38 -136. 2015.

Salgado, F.H.M. and Ximenes, P.A. Maize seed Germination treated with insecticides. Journal of Biotechnology and Biodiversity. 4: 49-53. 2013.

Sidra, A., Aisha, T., Muhammadu, F., Muhammadu, W., Arshad, A.S. and Schrish, S.Recent advances in molecular techniques for the identification of phytopathologenic fungal. Journal of plant interactions. 12 (1): 493-504. 2017.

Tonin, R.F.B. The physiological potential of hybrid corn treated with insecticides And store in two environmental conditions. Scientia Agropecuaria. 5: 7-10. 2014.

Trindade, L.C., Marques, E., Lopes, D.B. and Ferreira, M.S. Development of a Molecular method for detection and identification of Xanthomonas campestris pv. Viticola summa phytopathologica. 33 (1): 16-23. 2007.

Watanebe, T. Pictoral Atlas of Soil borne fungal plant pathogens and disease. Pp. 298. 2018.




DOI: https://doi.org/10.46676/ij-fanres.v5i3.311

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