Preparation and Optimization Formula of Whey Protein-Pectin Complex As Nanocapsules for Lemon (Citrus limon) Juice

Lailatul Azkiyah, Yukihiro Yamamoto, Shinjiro Ogita, Tomoyuki Yoshino

Abstract


Lemon (Citrus limon) juice was nanoencapsulated in whey protein concentrate (WPC)-pectin complex coacervates, and then dried using freeze-drying to produce solid nanocapsules. To optimize the nanoencapsulation condition, central composite design (CCD) of response surface methodology (RSM) was implemented. WPC (%), pectin (%) and pH level were taken as variables, while antioxidant activity and D-limonene content as responses. The optimized condition was in 6.0% WPC and 3.0% pectin at pH 3.1 by maximizing the antioxidant activity response in procedure. Scanning electron microscopy (SEM) and atomic force microscopy (AFM) images showed the morphology of lemon juice nanocapsules as spherical nanoparticles with an average size of 22.3 nm.

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References


[USDA] United States Department of Agriculture, Agricultural Research Service, “USDA National Nutrient Database for Standard Reference, Release 24,” 2011, Available from: http://www.ars.usda.gov/services/ docs.htm?docid=8964. Accessed 2012 April.

Robards, K., Antolovich, M., “Analytical chemistry of fruit bioflavonoids. A review,” Analyst, 122(1997), 11–34.

Klimek-Szczykutowicz, M., Szopa, A., Ekiert, H., “Citrus limon (Lemon) Phenomenon—A Review of the Chemistry, Pharmacological Properties, Applications in the Modern Pharmaceutical, Food, and Cosmetics Industries, and Biotechnological Studies. Review,” Plants, 9 (2020), 119.

Dosoky, N.S., Setzer, W.N., “Biological activities and safety of Citrus spp. Essential oils,” Int.J.Mol.Sci, 19 (2018), 1996.

S. K. Jawandha, H. Singh, A. Anita, J. Singh, “Effect of modified atmosphere packaging on storage of Baramasi lemon (Citrus limon (L.) Burm),” International Journal of Agriculture, Environment and Biotechnology, 7(3) (2014), 635-638.

A. A. Franke, R. V. Cooney, S. M. Henning, L. J. Custer, Bioavailability and antioxidant effects of orange juice components in humans,” Journal of Agriculture and Food Chemistry, 53 (13) (2005), 5170-5178.

J. Ubbink, J. Kruger, “Physical approaches for the delivery of active ingredients in food,” Trends Food Sci. Technol, 17 (2006), 244-254.

J. Weiss, P. Takhistov, J. McClements, “Functional materials in food nanotechnology,” J. Food Sci, 71 (2006), 107-116.

V. Nedovic, A. Kalusevic, V. Manojlovic, S. Levic, B. Bugarski, “An overview of encapsulation technologies for food applications,” Procedia Food Science, 1 (2011), 1806-1815.

A. Matalanis, O. G. Jones, D. J. McClements, “Structured biopolymer-based delivery systems for encapsulation, protection, and release of lipophilic compounds,” Food Hydrocolloids, 25 (8) (2011), 1865-1880.

S. Ghasemi, S. M. Jafari, E. Assadpour, M. Khomeiri, “Nanoencapsulation of D-limonene within nanocarriers produced by pectin-whey protein complexes,” Food Hydrocolloids, 77 (2018) 152-162.

S. L. Turgeon, C. Schmitt, C. Sanchez, “Protein-polysaccharide complexes and coacervates,” Curr.Opin, Colloid Interface Sci, 12 (2007), 166-178.

S. L. Turgeon, M. Beaulieu, C. Schmitt, C. Sanchez, “Protein-polysaccharide interactions: phase-ordering kinetics, thermodynamic and structural aspects,” Curr. Opin, Colloid Interface Sci, 8 (2003), 401–414.

C. G. De Kruif, R. Tuiner, “Polysaccharide protein interactions,” Food Hydrocollids,” 12 (2001), 555-563.

Priftis, D., Tirrell, M., “Phase behaviour and complex coacervation of aqueous polypeptide solutions,” Soft Matter, 8 (2012), 9396-9405.

T. Hatano, H. Kagawa, T. Yasuhara, T. Okuda, “Two new lavonoid and other contituents in licorice root: their relative astringency and scavenging effects,” Chem. Pharm. Bull, 36 (1988), 2090-2097.

Tisserand, R., Young, R., “Essential Oil Safety, 2nd ed,” Elsevier: New York, NY, USA (2014).

A. Q. Ye, “Complexation between milk proteins and polysaccharides via electrostatic interaction: principles and applications – a review,” International Journal of Food Science and Technology, 43:3 (2008), 406-415.

G. K. Bedie, S. L. Turgeon, J. Makhlouf, “Formation of native whey protein isolate-low methoxyl pectin complexes as a matrix for hydro-soluble food ingredient entrapmentin acidic foods,” Food Hydrocolloids, 22:5 (2008), 836-844.

A. Farrag, T. M. El-Messery, M. M. El-said, T. N. Soliman, H. M. F. El-Din, “Microencapsulation of grape phenolic compounds using whey protein as carrier vehicle,” J.Biol.Sci, 18:7 (2018), 373-380.




DOI: https://doi.org/10.46676/ij-fanres.v1i1.1

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