Chemical and microbiological quality of commercial fresh and frozen chicken drumstick in Umuhia, Nigeria


Judith Chukwuebinim Okolo, Jude Chukwuemeke Igborgbor, Unyimeabasi Effiong Anana, Gideon Ikechukwu Ogu


Abstract


Chicken meat, an excellent source of good quality proteins, is highly susceptible to microbial contaminations and heavy metals from feeds, processing, and retailing environments, thereby posing health risks to consumers. Therefore, the proximate composition, heavy metal concentrations, and microbial loads of commercial fresh and frozen chicken drumsticks, randomly sourced from poultry farms and groceries, respectively, within Umuahia metropolis, Abia State, Nigeria, were investigated to ascertain their qualities. A total of 40 (20 each of the fresh and frozen) chicken drumstick samples were processed and analyzed for proximate (moisture, fats, ash, proteins, fibers, and carbohydrates), heavy metal (cadmium, chromium, copper, manganese, nickel, zinc, and lead) contents, and microbial loads (total viable counts, Escherichia coli counts, Salmonella counts, Staphylococcus counts and fungal counts) and characterization using standard techniques. Results were analyzed statistically (p<0.01, 0.05). The fresh chicken drumstick samples had significantly higher (p<0.01, 0.05) levels of moisture (61.38 ± 0.34 %), proteins (22.30 ± 0.02 %), ash (5.27 ± 0.17 %) and microbial contents (0.24 × 102 CFU/g to 2.42 × 104 CFU/g), when compared with the frozen drumstick samples with significantly higher (p<0.01, 0.05) contents of fats (3.70 ± 0.13 %), carbohydrates (12.55 ± 0.27 %) and heavy metals (0.047 ± 0.017 mg/kg to 0.852 ± 0.456 mg/kg). This suggests that fresh chicken drumstick is more nutritionally beneficial, provided they are thoroughly treated with heat before consumption to prevent foodborne diseases from bacterial and fungal contaminations. Although the levels of heavy metals in both meat samples are below the toxic limits, consumers should be wary of bioaccumulation and bio-magnification of heavy metals when constantly exposed to them. Hence, constant surveillance of chicken meat is encouraged to monitor their exposure levels to these chemicals and biohazards.


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M. M. S. Saraiva, K. Lim, D. F. M. de-Monte, P. Emilia, N. Givosiez, L. N. R. A1ves, O. C. F. Neto, S. Kariuki, A. B. Junior, C. J. B. de-Oliveira, and W. A. Gebreyes, “Antimicrobial resistance in globalized food chain: a One Health perspective applied to the poultry industry,” Bra. J. Microbiol. Vol. 53, mo. 1, pp. 465-486, March 2022.

G. I. Ogu and F. I. Akinnibosun, “Health risks associated with heavy metals in commercial chicken meat via consumption within southern Nigeria,” Afr. J. Health, Safety and Environ. Vol. 1, np. 1, pp. 22-37, March 2020.

G. I. Ogu and F. I. Akinnibosun, “Occurrence of salmonella in raw chicken meat from retail equipment and environments in southern Nigeria open markets,” Not. Sci. Biol. Vol. 11, no. 2, pp. 175-182. June 2019

N. T. M. Elsharawy, “Some heavy metals residues in chicken meat and their edible offal in New Valley,” Suez Canal University, Faculty of Veterinary Medicine, Vol. 1 pp. 53 – 60, August 2015 [2nd Conference of Food Safety, Suez Canal University].

A. Filazi, B. Yurdakok-Dikmen, O. Kuzukiran and U. T. Sireli, “Chemical contaminants in poultry meat and products” In: Poultry Science. Intech Science Open. Pp. 171 – 190, February 2017.

H. U. Abdu and A. M. Abubakar, “Comparative bacteriological analysis of frozen and fresh chicken meats sold around Old Site Bayero University Kano”. BAJOPAS, Vol. 14, no. 1, pp. 258 – 262, June 2021.

M. Bas, A. S. Ersun and G. Kivanc, “The evaluation of food hygiene knowledge, attitudes and practice of food handlers in food business in Turkey,” J. Food Contr., Vol. 17, pp. 317-322, April2006.

L. R. Beuchat and J. H. Ryu, “Procedure handling and process practices” Emerg. Infect. Dis., Vol. 3, 459-465, December 1997.

L. Kozacinski, M. Hadaziosmanovic and N. Zdolec, “Microbiological quality of poultry meat on the Croatian market,” VeterinarskiArhiv” Vol. 76, no. 14, pp. 305-313, August 2006.

O. P. Osakue, J. O. Igene, P. A. Ebabhamiegbebho and S. E. Evivie, “Proximate analysis and microbial quality of ready to-eat (RTE) fried chicken parts,” J. Food Ind. Microb., Vol. 2, p. 107, March 2016.

P. R. Selvan, B. Narendra, S. Sureshkumar and V. Venkatamanujam, “Microbial quality of retail meat products available in Chennai City. Am. J. Food Technol., Vol. 2, no. 1, pp. 55-59, January 2007.

International Atomic Energy Agency (IAEA), “Irradiation of Poultry Meat and Its Products,” IAEA TECDOC-688, Vienna, 1993.

P. S. Mead, L. Slutsker, V. Dietz, L. F. McCaig, J. S. Bresee, C. Shapiro, P. M. Griffin and R. V. Tauxe, “Food-related illness and death in the United States,” Emerg. Infect. Dis. Vol. 5, no. 5, pp. 607-625, October 1999.

S. Chaillou, A. Chaulot-Talmon, H. Caekebeke, M. Cardinal, S. Christieans, C. Denis, M. H. Desmonts, X. Dousset, C. Feurer and E. Hamon “Origin and ecological selection of core and food specific bacterial communities associated with meat and seafood spoilage,” ISME J. Vol. 9, pp. 1105–1118, May 2015.

L. Holl, J. Behr, and R. F. Vogel, “Identification and growth dynamics of meat spoilage microorganisms in modified atmosphere packaged poultry meat by MALDI-TOFMS,” FoodMicrobiol., Vol. 60, pp. 84–91, December 2016.

I. E. Daniel, “Proximate composition and levels of trace metals in chicken meat consumed in Uyo Metropolis, Akwa Ibom State,” Annal. Food Sci. Technol. Vol. 16, no. 1, pp. 262-266, March 2015.

D. O. Nwude, P. A. C. Okoye and J. O. Babayemi, “Heavy metal levels in animal muscle tissue: A case study of Nigerian raised cattle,” Res J. Appl. Sci., Vol. 5, no. 2, pp. 146-150, February 2010.

A. F. Hasballah, “Evaluation of some heavy metals in cows meat and some chicken organs in Damietta Governorate markets and effect of the heat on their levels,” J. Env. Sci., Vol. 48, no. 3, pp. 131- 141, April 2019.

E. Kurnaz and A. Filazi “Determination of metal levels in the muscle tissue and livers of chickens,” Fresen Environ. Bull., Vol. 20, pp. 2896–2901, January 2011.

M. A. Mottalib, G. Zilani, T. I. Suman, T. Ahmed, and S. Islam, “Assessment of trace metals in consumer chickens in Bangladesh, JH & P Vol. 8, no. 20, pp.1-10, December 2018.

V. Goetting, K. A. Lee and L. A. Tell, “Pharmacokinetics of veterinary drugs in laying hens and residues in eggs: a review of the literature,” J. Vet. Pharmacol. Thera., Vol. 34, pp. 521-556, December2011.

R. S. Aulakh, J. P. S. Gill, J. S. Bedi, J. K. Sharma, B. S. Joia and H. W. Ockerman, “Organochlorine pesticide residues in poultry feed, chicken muscle and eggs at a poultry farm in Punjab, India,” J. Sci. Food Agric., Vol. 86, pp.741–744, April 2006.

V. Di Stefano and G. Avellone, “Food contaminants,” J. Food Stud. Vol. 3, pp. 88–102, December 2014.

F. S. Walaa and A. R. Amal, “Residues of some toxic heavy metals and trace elements in chicken eggs,” Zagazig Vet. J., Vol. 46, no. 1, pp. 8-16, March 2018.

C. Amos-Uhegbu, M. U. Igboekwe, G. U. Chukwu, K. O. Okengwu and T. K. Eke, “Hydrogen physical delineation and hydrogeochemical characterization of the aquifer systems in Umuahia-South Area, Southern Nigeria,” British J. Appl. Sci. Technol., Vol. 2, no. 4, pp. 406-432, December 2012.

G. I. Onwuka, “Food Analysis and Instrumentation: Theory and practices (second edition) Napthali prints, A division of HG Support Nigeria Limited Surulere Lagos, Nigeria. Pp 179 – 228, 2018.

C. M. A. Iwegbue, “Heavy metal composition of livers and kidneys of cattle from Southern Nigeria,” Vet. Arhiv., Vol. 78, pp. 401-410, October 2008.

G. I. Ogu, I. H. Madar, J. O. Okolo, E. M. Eze, S. Srinivasan and A. T. Iftikhar, “Exposure assessment of chicken meat to heavy metals and bacterial contaminations in Warri metropolis, Nigeria,” Int. J. Sci. Innovs., Vol. 3, no. 2, pp. 047-053, April 2018.

C. J. Alexopoulos and C. W. Mims, “Introductory Mycology,” 3rd Edition. John Wiley and sons, Newyork. P. 269, 979.

M. O. Fawole and B. A. Oso, “Laboratory Manual of Microbiology,” Spectrum books limited. P. 127, 1988.

J. G. Holt, N. R. Krieg, P. H. A. Sneath, J. T. Staley and S. T. Williams, “Bergey's Manual of Determinative Bacteriology. 9th edition, Lippincot: Williams and Wilkins. Philadelphia, USA. Pp. 131-542, 2002.

National Research Council (NRC), “Nutrient Requirements of Poultry,” 9th ed.; 8. Toxicity of Certain Inorganic Elements; National Academy Press: Washington, DC, USA, pp. 58-60, 1994.

T. Daszkiewicz, D. Kubiak and A. Panfil, “The effect of long storage on the qualityof meat (Longissimus thoraciset Lumborum) from female Roe Deer (Capreolus capreolus). J. Food Quality. Vol. 2018, pp. 1-7, October 2018.

C. Hammuel, I. O. Abdullahi, C. M. Z. Whong, K. B. Kadima and R. P. Enenya, “Assessment of proximate composition and bacteriological quality of some fresh meat sold in parts of Kaduna State, Nigeria. FUDMA J. Microbiol., Vol. 2, no. 1, pp. 26 – 31, June 2019.

C. Bristone, H. S. Abubakar, M. H. Badau, “The Proximate composition, heavy metals and microbial load of cow, goat and sheep offals sold in University of Maiduguri,” Int. J. Res. Stud. Agric. Sci., Vol. 4, no. 11, pp. 10-17, November 2018.

M. Jaishankar, T. Tseten, N. Anbalagan and B. B. Mathew, “Toxicity, mechanism and health effects of some heavy metals,” Interdiscip. Toxicol. Vol. 7, no. 2, pp. 60-72, 2014.

M. A. Korish and Y. A. Attia, Evaluation of heavy metal content in feed, litter, meat, meat products, liver, and table eggs of chickens,” Animals. Vol. 10, no. 727, pp. 1-22, April 2020.

O. O. Bello, T. K. Bello, O. A. Amolegbe and S. A. Sanwo, “Bacteriological assessment of raw meats sold in Lagos, Nigeria,” Sci. Agri., Vol. 16, no. 1, pp. 20-25, August 2016.

M. Kumari, A. Kumar and R. Gupta, “Enumeration of total viable and total coliform count in chicken meat and meat products using Tempo® System,” Haryana Vet. Vol. 57, no. 2, pp. 204-206, December2018.

M. O. Faruque, S. Mahmud, M. A. Munayem, R. Sultana, M. T. Molla, M. F. Ali, M. Wasim, S. Sarker and F. Z. Evamoni, “Bacteriological analysis and public health impact of broiler meat: a study on Nalitabari Paurosova, Sherpur, Bangladesh,” Adv. Microbiol., Vol. 9, pp. 581-601, July 2019.

S., Datta, A. Akter, I. G. Shah, K. Fatema, T. H. Islam, A. Bandyopadhyay, Z. Khan, and D. Biswas, “Microbiological quality assessment of raw meat and meat products and antibiotic susceptibility of isolated Staphylococcus aureus. Agric. Food Anal. Bacteriol., Vol. 2, pp. 187-194, November 2012.

P. Ramya, E. Tirupathi Reddy, A. Vijaya Kumar and N. Krishnaiah, “Study on microbiological analysis of raw meat. Int. J. Inst. Pharm. Life Sci., Vol. 5, pp. 1-9, 2015.

A. E. Morshdy, A. A. Abdel Samie, A. E. Tharwat, I. M. Elshorbagy and M. A. Hussein, “Prevalence of mould in chicken meat-cuts, giblets and products with immuno-affinity detection of aflatoxin residues,” Food Res., Vol. 5, no. 4, pp. 303 – 309, August 2021.

D. D. Yar, W. K. J. Kwenin, W. K. Zanu, G. I. Balali, E. K. Adepa and G. Francis, “Microbial qualityof frozen chicken parts from three import countries into the Kumasi metropolis of Ghana,” Microbiol. Res. J. Int., Vol. 31, no 6, pp. 43-53, September 2021.

G. I. Ogu, I. H. Mader, C. J. Igborgbor and J. C. Okolo, “Mycological quality of fresh and frozen chicken meat retailed within Warri metropolis, Delta State, Nigerian,” Jordan J. Bio. Sci. Vol. 10, no. 4, pp. 303-308, December 2017

International Commission on Microbiological Specifications for Foods (ICMSF) “Microorganism in Foods; Samples for Microbiological Analysis: Principles and Specific Applications, Recommendation of the International Commission on Microbiological Specification for Foods,” Association of Microbiological Societies, University of Toronto Press, Toronto. 1985.

G. I. Ogu, F. I. Akinnibosun, and T. O. Imade, “Phenotypic and molecular characterization of multidrug-resistant extended-spectrum beta-lactamase-producing Salmonella prevalent in raw chicken meat vended in Nigerian markets,” Ovidus Uni. Annal. Chem. Vol. 32, no. 1, pp. 76-84, June 2021.

G. I. Ogu, J. A. Odoh, J. C. Okolo, J. C., Igborgbor, and F. I. Akinnibosun, “Prevalence of multidrug-resistant extended spectrum beta-lactamase-producing Salmonella strains in commercial raw chicken meat,” Banglaesh J. Sci. Indust. Res. Vol. 56, no. 4, pp. 271 – 284, December 2021.

G. I. Ogu, J. C. Okolo, J. C., Igborgbor, and F. I. Akinnibosun, “Multidrug-resistance and biofilm formation profiles of Salmonella spp. isolated from raw chicken meat,” Novel Res. Microbiol. J. 5(2): 1214 – 1226. April 2021.

C. Rortana, H. Nguyen-Viet, S. Tum, F. Unger, S. Boqvist, S. Dang-Xuan, S. Koam, D. Grace, K. Osbjer, T. Heng, et al. Prevalence of Salmonella spp. and Staphylococcus aureus in chicken meat and pork from Cambodian Markets. Pathogens. Vol. 10, no. 5. P. 556, May 2021.

M. Marmion, M. T. Ferone, P. Whyte, A. G. M. Scannell, “The changing microbiome of poultry meat; from farm to ridge,” Food Microbiol., Vol. 99, e103823, 2021.

A. Chuku, L. B. Etim, A. B. Obande, B. E. Asikong and B. E. Sani, “Bacteriological quality of fresh raw beef and chevon retailed in Lafiya, Nigeria,” J. Microbiol. Res., 6(2), pp. 29-34, 2016.

A. Rouger, O. Tresse and M. Zagorec, “Review: Bacterial contaminants of poultry meat: sources, species, and dynamics,” J. Microorganisms. Vol. 5 no. 50, pp. 1-16, 2017.

D. K. Wardhana, A. E. P. Haskito, M. T. E. Purnama, D. A. Safitri and S. Annisa, “Detection of microbial contamination in chicken meat from local markets in Surabaya, East Java, Indonesia,” Vet. World. Vol. 14, no. 12, pp. 3138-3143, December 2021.

S. A. Zakki, R. Qureshi, A. Hussain, W. Ghias, M. Sharif and F. Ansari, “Microbial quality evaluation and prevalence of bacteria and fungus in different varieties of chicken meat in Lahore,” J. Pharm.Pharm. Sci., Vol. 5, no. 1, pp. 30-37, December 2017.

R. Yulistiani, D. Praseptiangga, D. Supyani and S. Sudibya. “Occurrences of Salmonella spp. and Escherichia coli in chicken meat, intestinal contents and rinse water at slaughtering place from traditional market in Surabaya, Indonesia,” Vol. 633. In: IOP Conference Series: Materials Science and Engineering. P. 012007, 2019.

D. K. Wardhana, M. H. Effendi, N. Harijani and O. Hong- Kean, “Detection of extended-spectrum beta-lact¬amase (ESBL) producing Escherichia coli in meat chicken from traditional market in Surabaya, East Java, Indonesia. Indian. J. Public Health Res. Dev. Vol. 11, no. 1, pp. 1353-1357, 2020.

Mashak, Z, “Prevalence and antibiotic resistance of Escherichia coli O157: H7 isolated from raw meat samples of ruminants and poultry,” J. Food Nutr. Res., Vol. 6, no. 2 96-102, 2018.

M. S. Rani, I. H. Madar, H. Al-Ssadh, G. I. Ogu and I. A. Tayubi, “Biochemical and phenotypic profiling of Bacillus clausii: a potent commercial probiotic. Int. J. Sci. Innov. Vol. 5, no. 03, pp. 99-106, August 2018.

A. W. Abd El-Ghany, “Pseudomonas areuginosa infection of avian origin: zoonosis and one health implications,” Vet. World. Vol. 14, no. 8, pp. 2155 – 2159, August 2021.

E. Madoroba, K. Magwedere, N. S. Chaora, I. Matle, F. Muchadeyi, M. A. Mathole and R. Pierneef, “Microbial communities of meat and meat products: an exploratory analysis of the product quality and safety at selected enterprises in South Africa,” Microorganisms. Vol. 9, no. 507. February 2021

F. Khalafalla, H. Ali, and A. El-Fouley, “Microbiological evaluation of chicken meat products,’ J. Vet. Med. Res., Vol. 26, no. 2, pp. 151-163, 2019.

Yahya, F., Ting, H., T. 2020. Effect of Different Ratios of Chicken Meat to Fresh Osyter Mushroom (Pleurotus Sajor-Caju) on The Physicochemical Properties And Sensory Acceptability of Sausages. International Journal of Food, Agriculture, and Natural Resources. Vol 1 (1):7-14.




DOI: https://doi.org/10.46676/ij-fanres.v3i3.113

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